Application Note
Purification process optimization of Fab fragments
Fab antibody fragments have been increasingly used for therapeutic applications. Expressed at high yield, they are very specific and lack the Fc portion of the molecule that may lead to undesirable immunological reactions into patients.
This application describes an accelerated three-step purification process for a fragment of antibody (FAB) that was developed on AcroPrep™ ScreenExpert plates and then transferred and scaled to column chromatography using PRC prepacked columns. The entire process was completed in less than 12 days leading to a 99% pure fragment and less than 30ppm of HCP.
