Application Note
Production of Human Stem Cells in Single-Use Vessels
Although successful expansion of mesenchymal stem cells (MSCs) in vitro has been well established, the large clinical-scale production of MSCs remains a bottleneck, potentially limiting clinical applications. In this study, the success of large clinical-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial single-use vessel at 3.75 L working volume is demonstated. The vessel offers a precision controlled environment for the ideal growth of stem cells under simulated physiological conditions.
With clinical relevance in mind, every cell culture step from T-flask to shake flask to bioreactor was conducted strictly using single-use consumables.
