Research Study
Expansion of Mesenchymal Stem Cells in Single-Use Bioreactors
Stem cell-based regenerative medicine has great potential to advance the therapeutic treatment of human diseases. Among the various stem cell platforms, mesenchymal stem cells (MSCs) represent one of the most promising options. Although successful expansion of MSCs in vitro has been well-established, higher-yield, billion-cell expansion of MSCs remains a bottleneck.
In this study, we successfully demonstrated large-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial, single-use vessel at 3.75 L scale. Specific properties of the stem cell were maintained, as evidenced by stem cell marker assays and differentiation assays performed at the conclusion of the run.
