Bio-Rad Laboratories Inc has released three new StarBright dyes designed for flow cytometry applications.
StarBright Violet 570, 670, and 710 dyes were developed to provide improved brightness and precise excitation and emission spectra, with Bio-Rad saying those characteristics make them suitable for use in multicolor flow cytometry panels.
The dyes offer researchers a range of fluorescent nanoparticles conjugated to highly validated flow antibodies, said the life sciences research and clinical diagnostics product developer.
“The new StarBright dyes offer improved brightness when compared to other available dyes at similar wavelengths, and they may be used with a wide range of flow cytometers,” said Mike Blundell, PhD, Bio-Rad product manager, flow cytometry, Life Science Group. “The unique profile of the dyes offers enhanced resolution of rare populations and low-density antigens as well as consistent and reproducible staining.”
The firm outlined what it sees as the key benefits of the dyes:
They are compatible with new and existing protocols and suitable for use in multiplex panels
They are resistant to photobleaching and highly stable with minimal lot-to-lot variation
They offer reliable Förster resonance energy transfer (FRET) for reproducible data
They work with common staining buffers, including special polymer dye staining buffers, for easy integration into multicolor panels
They ensure no loss of signal in fixation
They offer narrow excitation and emission profiles for improved resolution in new and existing multicolor flow cytometry experiments